NODES: Network of Optimal Dynamic Energy Signatures
NODES: Network of Optimal Dynamic Energy Signatures
Summary
The Network of Optimal Dynamic Energy Signatures (NODES) program aims to develop a groundbreaking deep learning tool, informed by the principles of biophysics, that can analyze vast numbers of protein sequences and predict their biological functions by identifying telltale patterns of protein movement.
With this program, DARPA seeks to characterize the function of potential biological threats within one hour, expanding threat assessment accuracy and speed exponentially. Speed is crucial for developing effective medical countermeasures for warfighters and for staying ahead of advancements in biotechnology that could lead to new and emerging biothreats.
Additionally, NODES will provide a secure system for classifying and monitoring biological threats, which is vital for intelligence agencies to enhance bio-surveillance.
Building protein understanding in motion
Folding landscape of disordered proteins on the surface of protein supercomplexes. Source: Jon Nyugen, Arizona State University
Folding
Protein sequences encode more than stationary structures seen in databanks, they encode an ensemble of molecular movements the landscape of which determines functions.
Tracking these functions offer a new lens at evolution and will take us in realms of engineering protein movements beyond nature.
Binding of chemokine proteins on surface of the SARS-CoV2 ChAdOX vaccine vector. Source: Chun Kit Chan, Arizona State University
Binding
Diffusion-driven protein-protein interactions offer rate-determining bottlenecks in multiple biological functionalities like signaling and recognition.
These pathways can be engineered to study pathogenicity and generation of medical countermeasures.
Proton-motive force driven coupled rotation in integral-membrane molecular motors. Source: Mrinal Shekhar, Broad Institute
Cooperativity
Beyond internal dynamics, proteins functions depend on modifications and environmental changes through cooperative or allosteric coupling that can also be chemo-mechanical.
Cooperativity controls protein movements in cellular environments and can be controlled to deliver biomanufacturing benefits.
Event
NODES: Proposers Day Aug. 1, 2025 1 - 5 p.m. ET Executive Conference Center Arlington, Va.
